By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-β-D- thiogalactopyranoside)-inducible promoter.
|Number of pages||4|
|Journal||Applied and Environmental Microbiology|
|Publication status||Published - 1 Apr 2007|