A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression

Reindert Nijland; Jan Willem Veening; Oscar P. Kuipers

doi:10.1128/AEM.02266-06

A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression

Reindert Nijland, Jan Willem Veening, Oscar P. Kuipers^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-β-D- thiogalactopyranoside)-inducible promoter.

Original language	English
Pages (from-to)	2390-2393
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	73
Issue number	7
DOIs	https://doi.org/10.1128/AEM.02266-06
Publication status	Published - 1 Apr 2007
Externally published	Yes

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abstract = "By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-β-D- thiogalactopyranoside)-inducible promoter.",

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AU - Veening, Jan Willem

AU - Kuipers, Oscar P.

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AB - By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-β-D- thiogalactopyranoside)-inducible promoter.

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DO - 10.1128/AEM.02266-06

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JO - Applied and Environmental Microbiology

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A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression

Abstract

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