A critical issue in model-based inference for studying trait-based community assembly and a solution

Cajo J.F. ter Braak*, Pedro Peres-Neto, Stéphane Dray

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)

Abstract

Statistical testing of trait-environment association from data is a challenge as there is no common unit of observation: the trait is observed on species, the environment on sites and the mediating abundance on species-site combinations. A number of correlation-based methods, such as the community weighted trait means method (CWM), the fourth-corner correlation method and the multivariate method RLQ, have been proposed to estimate such trait-environment associations. In these methods, valid statistical testing proceeds by performing two separate resampling tests, one sitebased and the other species-based and by assessing significance by the largest of the two p-values (the pmax test). Recently, regression-based methods using generalized linear models (GLM) have been proposed as a promising alternative with statistical inference via site-based resampling. We investigated the performance of this new approach along with approaches that mimicked the pmax test using GLM instead of fourth-corner. By simulation using models with additional random variation in the species response to the environment, the site-based resampling tests using GLM are shown to have severely inflated type I error, of up to 90%, when the nominal level is set as 5%. In addition, predictive modelling of such data using site-based cross-validation very often identified trait-environment interactions that had no predictive value. The problem that we identify is not an ``omitted variable bias'' problem as it occurs even when the additional random variation is independent of the observed trait and environment data. Instead, it is a problem of ignoring a random effect. In the same simulations, the GLM-based pmax test controlled the type I error in all models proposed so far in this context, but still gave slightly inflated error in more complex models that included both missing (but important) traits and missing (but important) environmental variables. For screening the importance of single trait-environment combinations, the fourth-corner test is shown to give almost the same results as the GLM-based tests in far less computing time.

Original languageEnglish
Article numbere2885
JournalPeerJ
Volume5
DOIs
Publication statusPublished - 2017

Keywords

  • Community composition
  • Compositional count data
  • Fourthcorner problem
  • Generalized linear models
  • Log-linear model
  • Negative-binomial response
  • Poisson regression
  • Trait-environment association

Fingerprint

Dive into the research topics of 'A critical issue in model-based inference for studying trait-based community assembly and a solution'. Together they form a unique fingerprint.

Cite this