A comparison of liquid chromatography, capillary electrophoresis, and mass spectrometry methods to determine xyloglucan structures in black currants

H. Hilz; L.E. de Jong; M.A. Kabel; H.A. Schols; A.G.J. Voragen

doi:10.1016/j.chroma.2006.08.024

A comparison of liquid chromatography, capillary electrophoresis, and mass spectrometry methods to determine xyloglucan structures in black currants

H. Hilz, L.E. de Jong, M.A. Kabel, H.A. Schols, A.G.J. Voragen

Research output: Contribution to journal › Article › Academic › peer-review

50 Citations (Scopus)

Abstract

Different separation (HPAEC, RP¿HPLC, CE) and identification (MALDI¿TOF¿MS, ESI¿MSn) techniques were compared to analyse oligosaccharides obtained after incubation of xyloglucan with endo-glucanase. It was possible to analyse xyloglucan oligosaccharides with each technique. Several techniques, including off line (HPAEC¿MALDI¿TOF¿MS) or online (CE¿ESI¿MSn, RP¿HPLC¿ESI¿MSn) connection provided complementary information on xyloglucan structure. Online CE¿MS and RP¿HPLC¿MS are described for the first time in xyloglucan analysis. Advantages and disadvantages of the techniques for different purposes such as structural characterisation of oligosaccharides or oligosaccharide profiling are discussed. Black currant xyloglucans had a rather simple XXXG-type structure with galactose and fucose containing side chains.

Original language	English
Pages (from-to)	275-286
Journal	Journal of Chromatography. A, Including electrophoresis and other separation methods
Volume	1133
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2006.08.024
Publication status	Published - 2006

Keywords

plant-cell walls
cultured sycamore cells
cellulose interactions
covalent linkage
fruit xyloglucan
side-chain
oligosaccharides
polysaccharides
hemicellulose
spectroscopy

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10.1016/j.chroma.2006.08.024

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Hilz, H., de Jong, L. E., Kabel, M. A., Schols, H. A., & Voragen, A. G. J. (2006). A comparison of liquid chromatography, capillary electrophoresis, and mass spectrometry methods to determine xyloglucan structures in black currants. Journal of Chromatography. A, Including electrophoresis and other separation methods, 1133(1-2), 275-286. https://doi.org/10.1016/j.chroma.2006.08.024

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title = "A comparison of liquid chromatography, capillary electrophoresis, and mass spectrometry methods to determine xyloglucan structures in black currants",

abstract = "Different separation (HPAEC, RP¿HPLC, CE) and identification (MALDI¿TOF¿MS, ESI¿MSn) techniques were compared to analyse oligosaccharides obtained after incubation of xyloglucan with endo-glucanase. It was possible to analyse xyloglucan oligosaccharides with each technique. Several techniques, including off line (HPAEC¿MALDI¿TOF¿MS) or online (CE¿ESI¿MSn, RP¿HPLC¿ESI¿MSn) connection provided complementary information on xyloglucan structure. Online CE¿MS and RP¿HPLC¿MS are described for the first time in xyloglucan analysis. Advantages and disadvantages of the techniques for different purposes such as structural characterisation of oligosaccharides or oligosaccharide profiling are discussed. Black currant xyloglucans had a rather simple XXXG-type structure with galactose and fucose containing side chains.",

keywords = "plant-cell walls, cultured sycamore cells, cellulose interactions, covalent linkage, fruit xyloglucan, side-chain, oligosaccharides, polysaccharides, hemicellulose, spectroscopy",

author = "H. Hilz and {de Jong}, L.E. and M.A. Kabel and H.A. Schols and A.G.J. Voragen",

year = "2006",

doi = "10.1016/j.chroma.2006.08.024",

language = "English",

volume = "1133",

pages = "275--286",

journal = "Journal of Chromatography. A, Including electrophoresis and other separation methods",

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Hilz, H, de Jong, LE, Kabel, MA , Schols, HA & Voragen, AGJ 2006, 'A comparison of liquid chromatography, capillary electrophoresis, and mass spectrometry methods to determine xyloglucan structures in black currants', Journal of Chromatography. A, Including electrophoresis and other separation methods, vol. 1133, no. 1-2, pp. 275-286. https://doi.org/10.1016/j.chroma.2006.08.024

A comparison of liquid chromatography, capillary electrophoresis, and mass spectrometry methods to determine xyloglucan structures in black currants. / Hilz, H.; de Jong, L.E.; Kabel, M.A. et al.
In: Journal of Chromatography. A, Including electrophoresis and other separation methods, Vol. 1133, No. 1-2, 2006, p. 275-286.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - A comparison of liquid chromatography, capillary electrophoresis, and mass spectrometry methods to determine xyloglucan structures in black currants

AU - Hilz, H.

AU - de Jong, L.E.

AU - Kabel, M.A.

AU - Schols, H.A.

AU - Voragen, A.G.J.

PY - 2006

Y1 - 2006

N2 - Different separation (HPAEC, RP¿HPLC, CE) and identification (MALDI¿TOF¿MS, ESI¿MSn) techniques were compared to analyse oligosaccharides obtained after incubation of xyloglucan with endo-glucanase. It was possible to analyse xyloglucan oligosaccharides with each technique. Several techniques, including off line (HPAEC¿MALDI¿TOF¿MS) or online (CE¿ESI¿MSn, RP¿HPLC¿ESI¿MSn) connection provided complementary information on xyloglucan structure. Online CE¿MS and RP¿HPLC¿MS are described for the first time in xyloglucan analysis. Advantages and disadvantages of the techniques for different purposes such as structural characterisation of oligosaccharides or oligosaccharide profiling are discussed. Black currant xyloglucans had a rather simple XXXG-type structure with galactose and fucose containing side chains.

AB - Different separation (HPAEC, RP¿HPLC, CE) and identification (MALDI¿TOF¿MS, ESI¿MSn) techniques were compared to analyse oligosaccharides obtained after incubation of xyloglucan with endo-glucanase. It was possible to analyse xyloglucan oligosaccharides with each technique. Several techniques, including off line (HPAEC¿MALDI¿TOF¿MS) or online (CE¿ESI¿MSn, RP¿HPLC¿ESI¿MSn) connection provided complementary information on xyloglucan structure. Online CE¿MS and RP¿HPLC¿MS are described for the first time in xyloglucan analysis. Advantages and disadvantages of the techniques for different purposes such as structural characterisation of oligosaccharides or oligosaccharide profiling are discussed. Black currant xyloglucans had a rather simple XXXG-type structure with galactose and fucose containing side chains.

KW - plant-cell walls

KW - cultured sycamore cells

KW - cellulose interactions

KW - covalent linkage

KW - fruit xyloglucan

KW - side-chain

KW - oligosaccharides

KW - polysaccharides

KW - hemicellulose

KW - spectroscopy

U2 - 10.1016/j.chroma.2006.08.024

DO - 10.1016/j.chroma.2006.08.024

M3 - Article

SN - 0021-9673

VL - 1133

SP - 275

EP - 286

JO - Journal of Chromatography. A, Including electrophoresis and other separation methods

JF - Journal of Chromatography. A, Including electrophoresis and other separation methods

IS - 1-2

ER -

A comparison of liquid chromatography, capillary electrophoresis, and mass spectrometry methods to determine xyloglucan structures in black currants

Abstract

Keywords

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