A Comparison of In Vitro and In Vivo Asexual Embryogenesis

Melanie Hand, S.C. de Vries, Anna Koltunow

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

7 Citations (Scopus)

Abstract

In plants, embryogenesis generally occurs through the sexual process of double fertilization, which involves a haploid sperm cell fusing with a haploid egg cell to ultimately give rise to a diploid embryo. Embryogenesis can also occur asexually in the absence of fertilization, both in vitro and in vivo. Somatic or gametic cells are able to differentiate into embryos in vitro following the application of plant growth regulators or stress treatments. Asexual embryogenesis also occurs naturally in some plant species in vivo, from either ovule cells as part of a process defined as apomixis, or from somatic leaf tissue in other species. In both in vitro and in vivo asexual embryogenesis, the embryo precursor cells must attain an embryogenic fate without the act of fertilization. This review compares the processes of in vitro and in vivo asexual embryogenesis including what is known regarding the genetic and epigenetic regulation of each process, and considers how the precursor cells are able to change fate and adopt an embryogenic pathway.
LanguageEnglish
Title of host publicationIn Vitro Embryogenesis in Higher Plants
EditorsMaria Antonietta Germanà, Maurizio Lambardi
PublisherHumana Press
Pages3-23
Volume1359
ISBN (Electronic)9781493930616
ISBN (Print)9781493930609
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1359
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

embryogenesis
embryo (plant)
fertilization (reproduction)
haploidy
cells
apomixis
in vitro fertilization
ova
plant growth substances
ovules
epigenetics
diploidy
spermatozoa
leaves

Cite this

Hand, M., de Vries, S. C., & Koltunow, A. (2016). A Comparison of In Vitro and In Vivo Asexual Embryogenesis. In M. A. Germanà, & M. Lambardi (Eds.), In Vitro Embryogenesis in Higher Plants (Vol. 1359, pp. 3-23). (Methods in Molecular Biology; Vol. 1359). Humana Press. https://doi.org/10.1007/978-1-4939-3061-6_1
Hand, Melanie ; de Vries, S.C. ; Koltunow, Anna. / A Comparison of In Vitro and In Vivo Asexual Embryogenesis. In Vitro Embryogenesis in Higher Plants. editor / Maria Antonietta Germanà ; Maurizio Lambardi. Vol. 1359 Humana Press, 2016. pp. 3-23 (Methods in Molecular Biology).
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Hand, M, de Vries, SC & Koltunow, A 2016, A Comparison of In Vitro and In Vivo Asexual Embryogenesis. in MA Germanà & M Lambardi (eds), In Vitro Embryogenesis in Higher Plants. vol. 1359, Methods in Molecular Biology, vol. 1359, Humana Press, pp. 3-23. https://doi.org/10.1007/978-1-4939-3061-6_1

A Comparison of In Vitro and In Vivo Asexual Embryogenesis. / Hand, Melanie; de Vries, S.C.; Koltunow, Anna.

In Vitro Embryogenesis in Higher Plants. ed. / Maria Antonietta Germanà; Maurizio Lambardi. Vol. 1359 Humana Press, 2016. p. 3-23 (Methods in Molecular Biology; Vol. 1359).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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Hand M, de Vries SC, Koltunow A. A Comparison of In Vitro and In Vivo Asexual Embryogenesis. In Germanà MA, Lambardi M, editors, In Vitro Embryogenesis in Higher Plants. Vol. 1359. Humana Press. 2016. p. 3-23. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3061-6_1