A common carp (Cyprinus carpio L.) leucocyte cell line shares morphological and functional characteristics with macrophages.

F.A.A. Weyts, J.H.W.M. Rombout, G. Flik, B.M.L. Verburg-van Kemenade

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50 Citations (Scopus)

Abstract

A carp leucocyte cell line (CLC), originating from peripheral blood, was characterised to assess its suitability for studies into carp macrophage functions. The cells reacted with a monoclonal antibody raised against carp head kidney macrophages. Other macrophage characteristics observed were: binding to an antibody against complement component C3, suggesting that the cells expose a complement receptor; the presence of acid-phosphatase positive cytoplasmic granules; the capacity to phagocytose sheep red blood cells and the upregulation of respiratory burst activity by phorbol myristate acetate, by lipopolysaccharide and by co-culture with carp peripheral blood leucocytes. Moreover, CLC cells produced and secreted an interleukin-1-like factor, as culture supernatants stimulated proliferation of carp peripheral blood leucocytes and proliferation of the interleukin-1-dependent mouse D10(N4)M T cell line. It is concluded that the CLC cell line is suitable for studies on macrophage activation, respiratory burst activity and may also be useful as a source of interleukin-1.
Original languageEnglish
Pages (from-to)123-133
JournalFish and Shellfish Immunology
Volume7
DOIs
Publication statusPublished - 1997

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