A closer look to cell structural barriers affecting starch digestibility in beans

Ana M. Rovalino-Córdova*, Vincenzo Fogliano, Edoardo Capuano

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Isolated bean cells were used to understand the contribution of cell wall and cytoplasmic matrix on starch digestibility. Cotyledon cells were treated enzymatically and mechanically to reduce the level of cell intactness. SEM and chemical characterization revealed that enzymatic treatment modified cell wall thickness and porosity without altering the cytoplasmic matrix, whereas mechanical treatment completely disrupted cell structure. Decreasing cell intactness increased the rate but not the extent of starch digestion in-vitro. It was concluded that cell wall serves as a permeable barrier limiting the access of digestive enzymes. Cytoplasmic matrix, on the other hand, reduced further the accessibility of amylase to starch affecting its hydrolysis rate. In addition, it was proven that cell structural changes, if any, occurring during digestion had no effect on starch hydrolysis.
Original languageEnglish
Pages (from-to)994-1002
JournalCarbohydrate Polymers
Volume181
DOIs
Publication statusPublished - 1 Feb 2018

Keywords

  • Bioaccessibility
  • Cell walls
  • In-vitro starch digestion
  • Kidney beans
  • Microstructure

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