A cascade extraction of active phycocyanin and fatty acids from Galdieria phlegrea

Paola Imbimbo, Valeria Romanucci, Antonino Pollio, Carolina Fontanarosa, Angela Amoresano, Armando Zarrelli, Giuseppe Olivieri*, Daria Maria Monti

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

The setup of an economic and sustainable method to increase the production and commercialization of products from microalgae, beyond niche markets, is a challenge. Here, a cascade approach has been designed to optimize the recovery of high valuable bioproducts starting from the wet biomass of Galdieria phlegrea. This unicellular thermo-acidophilic red alga can accumulate high-value compounds and can live under conditions considered hostile to most other species. Extractions were performed in two sequential steps: a conventional high-pressure procedure to recover phycocyanins and a solvent extraction to obtain fatty acids. Phycocyanins were purified to the highest purification grade reported so far and were active as antioxidants on a cell-based model. Fatty acids isolated from the residual biomass contained high amount of PUFAs, more than those recovered from the raw biomass. Thus, a simple, economic, and high effective procedure was set up to isolate phycocyanin at high purity levels and PUFAs.

Original languageEnglish
Pages (from-to)9455-9464
JournalApplied Microbiology and Biotechnology
Volume103
Issue number23-24
DOIs
Publication statusPublished - Dec 2019

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Phycocyanin
Biomass
Fatty Acids
Economics
Microalgae
Rhodophyta
Antioxidants
Pressure

Keywords

  • Biorefinery approach
  • Galdieria phlegrea
  • Lipids
  • Phycocyanin

Cite this

Imbimbo, P., Romanucci, V., Pollio, A., Fontanarosa, C., Amoresano, A., Zarrelli, A., ... Monti, D. M. (2019). A cascade extraction of active phycocyanin and fatty acids from Galdieria phlegrea. Applied Microbiology and Biotechnology, 103(23-24), 9455-9464. https://doi.org/10.1007/s00253-019-10154-0
Imbimbo, Paola ; Romanucci, Valeria ; Pollio, Antonino ; Fontanarosa, Carolina ; Amoresano, Angela ; Zarrelli, Armando ; Olivieri, Giuseppe ; Monti, Daria Maria. / A cascade extraction of active phycocyanin and fatty acids from Galdieria phlegrea. In: Applied Microbiology and Biotechnology. 2019 ; Vol. 103, No. 23-24. pp. 9455-9464.
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Imbimbo, P, Romanucci, V, Pollio, A, Fontanarosa, C, Amoresano, A, Zarrelli, A, Olivieri, G & Monti, DM 2019, 'A cascade extraction of active phycocyanin and fatty acids from Galdieria phlegrea', Applied Microbiology and Biotechnology, vol. 103, no. 23-24, pp. 9455-9464. https://doi.org/10.1007/s00253-019-10154-0

A cascade extraction of active phycocyanin and fatty acids from Galdieria phlegrea. / Imbimbo, Paola; Romanucci, Valeria; Pollio, Antonino; Fontanarosa, Carolina; Amoresano, Angela; Zarrelli, Armando; Olivieri, Giuseppe; Monti, Daria Maria.

In: Applied Microbiology and Biotechnology, Vol. 103, No. 23-24, 12.2019, p. 9455-9464.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A cascade extraction of active phycocyanin and fatty acids from Galdieria phlegrea

AU - Imbimbo, Paola

AU - Romanucci, Valeria

AU - Pollio, Antonino

AU - Fontanarosa, Carolina

AU - Amoresano, Angela

AU - Zarrelli, Armando

AU - Olivieri, Giuseppe

AU - Monti, Daria Maria

PY - 2019/12

Y1 - 2019/12

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AB - The setup of an economic and sustainable method to increase the production and commercialization of products from microalgae, beyond niche markets, is a challenge. Here, a cascade approach has been designed to optimize the recovery of high valuable bioproducts starting from the wet biomass of Galdieria phlegrea. This unicellular thermo-acidophilic red alga can accumulate high-value compounds and can live under conditions considered hostile to most other species. Extractions were performed in two sequential steps: a conventional high-pressure procedure to recover phycocyanins and a solvent extraction to obtain fatty acids. Phycocyanins were purified to the highest purification grade reported so far and were active as antioxidants on a cell-based model. Fatty acids isolated from the residual biomass contained high amount of PUFAs, more than those recovered from the raw biomass. Thus, a simple, economic, and high effective procedure was set up to isolate phycocyanin at high purity levels and PUFAs.

KW - Biorefinery approach

KW - Galdieria phlegrea

KW - Lipids

KW - Phycocyanin

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DO - 10.1007/s00253-019-10154-0

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JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

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Imbimbo P, Romanucci V, Pollio A, Fontanarosa C, Amoresano A, Zarrelli A et al. A cascade extraction of active phycocyanin and fatty acids from Galdieria phlegrea. Applied Microbiology and Biotechnology. 2019 Dec;103(23-24):9455-9464. https://doi.org/10.1007/s00253-019-10154-0