A broader role for AmyR in Aspergillus niger: regulation of the utilisation of d-glucose or d-galactose containing oligo- and polysaccharides

P.A. vanKuyk, J.A.E. Benen, H.A.B. Wosten, J.A.G.M. de Visser, R.P. de Vries

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39 Citations (Scopus)

Abstract

AmyR is commonly considered a regulator of starch degradation whose activity is induced by the presence of maltose, the disaccharide building block of starch. In this study, we demonstrate that the role of AmyR extends beyond starch degradation. Enzyme activity assays, genes expression analysis and growth profiling on d-glucose- and d-galactose-containing oligo- and polysaccharides showed that AmyR regulates the expression of some of the Aspergillus niger genes encoding alpha- and beta-glucosidases, alpha- and beta- galactosidases, as well as genes encoding alpha-amlyases and glucoamylases. In addition, we provide evidence that d-glucose or a metabolic product thereof may be the inducer of the AmyR system in A. niger and not maltose, as is commonly assumed.
Original languageEnglish
Pages (from-to)285-293
JournalApplied Microbiology and Biotechnology
Volume93
Issue number1
DOIs
Publication statusPublished - 2012

Keywords

  • heterologous protein-production
  • transcriptional activator xlnr
  • glucoamylase production
  • alpha-galactosidase
  • gene-expression
  • encoding genes
  • amylase genes
  • degradation
  • oryzae
  • growth

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