A Bifidobacterial pilus-associated protein promotes colonic epithelial proliferation

Mary O'Connell Motherway*, Aileen Houston, Grace O'Callaghan, Justus Reunanen, Frances O'Brien, Tara O'Driscoll, Patrick G. Casey, Willem M. de Vos, Douwe van Sinderen, Fergus Shanahan

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

Development of the human gut microbiota commences at birth, with certain bifidobacterial species representing dominant and early colonisers of the newborn gastrointestinal tract. The molecular basis of Bifidobacterium colonisation, persistence and presumed communication with the host has remained obscure. We previously identified tight adherence (Tad) pili from Bifidobacterium breve UCC2003 as an essential colonisation factor. Here, we demonstrate that bifidobacterial Tad pili also promote in vivo colonic epithelial proliferation. A significant increase in cell proliferation was detectable 5 days postadministration of B. breve UCC2003. Using advanced functional genomic approaches, bacterial strains either (a) producing the Tad2003 pili or (b) lacking the TadE or TadF pseudopilins were created. Analysis of the ability of these mutant strains to promote epithelial cell proliferation in vivo demonstrated that the pilin subunit, TadE, is the bifidobacterial molecule responsible for this proliferation response. These findings were confirmed in vitro using purified TadE protein. Our data imply that bifidobacterial Tad pili may contribute to the maturation of the naïve gut in early life through the production of a specific scaffold of extracellular protein structures, which stimulate growth of the neonatal mucosa.

Original languageEnglish
Pages (from-to)287-301
JournalMolecular Microbiology
Volume111
Issue number1
Early online date23 Oct 2018
DOIs
Publication statusPublished - Jan 2019

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