A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression.

I. Vijn; H. Christiansen; P. Lauridsen; I. Kardailsky; H.J. Quandt; I. Broer; J. Drenth; E. Ostergaard Jensen; A. van Kammen; T. Bisseling

doi:10.1007/BF00032670

A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression.

I. Vijn, H. Christiansen, P. Lauridsen, I. Kardailsky, H.J. Quandt, I. Broer, J. Drenth, E. Ostergaard Jensen, A. van Kammen, T. Bisseling

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

ENOD12 is one of the first nodulin genes expressed upon inoculation with Rhizobium and also purified Nod factors are able to induce ENOD12 expression. The ENOD12 gene family in pea (Pisum sativum) has two members. A cDNA clone representing PsENOD12A [26] and a PsENOD12B genomic clone [7] have been previously described. The isolation and characterization of a PsENOD12A genomic clone is presented in this paper. By using a Vicia hirsuta-Agrobacterium rhizogenes transformation system it is shown that both genes have a similar expression pattern in transgenic V. hirsuta root nodules. Promoter analyses of both PsENOD12 promoters showed that the 200 bp immediately upstream of the transcription start are sufficient to direct nodule-specific and Nod factor-induced gene expression.

Original language	English
Pages (from-to)	1103-1110
Journal	Plant Molecular Biology
Volume	28
DOIs	https://doi.org/10.1007/BF00032670
Publication status	Published - 1995

Keywords

ENOD12
gene regulation
hairy roots
Rhizobium
transgenic root nodules
Vicia hirsuta

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10.1007/BF00032670

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@article{93d5a2fddf0643e281c26507649061b1,

title = "A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression.",

abstract = "ENOD12 is one of the first nodulin genes expressed upon inoculation with Rhizobium and also purified Nod factors are able to induce ENOD12 expression. The ENOD12 gene family in pea (Pisum sativum) has two members. A cDNA clone representing PsENOD12A [26] and a PsENOD12B genomic clone [7] have been previously described. The isolation and characterization of a PsENOD12A genomic clone is presented in this paper. By using a Vicia hirsuta-Agrobacterium rhizogenes transformation system it is shown that both genes have a similar expression pattern in transgenic V. hirsuta root nodules. Promoter analyses of both PsENOD12 promoters showed that the 200 bp immediately upstream of the transcription start are sufficient to direct nodule-specific and Nod factor-induced gene expression.",

keywords = "ENOD12, gene regulation, hairy roots, Rhizobium, transgenic root nodules, Vicia hirsuta",

author = "I. Vijn and H. Christiansen and P. Lauridsen and I. Kardailsky and H.J. Quandt and I. Broer and J. Drenth and {Ostergaard Jensen}, E. and {van Kammen}, A. and T. Bisseling",

year = "1995",

doi = "10.1007/BF00032670",

language = "English",

volume = "28",

pages = "1103--1110",

journal = "Plant Molecular Biology",

issn = "0167-4412",

publisher = "Springer",

}

TY - JOUR

T1 - A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression.

AU - Vijn, I.

AU - Christiansen, H.

AU - Lauridsen, P.

AU - Kardailsky, I.

AU - Quandt, H.J.

AU - Broer, I.

AU - Drenth, J.

AU - Ostergaard Jensen, E.

AU - van Kammen, A.

AU - Bisseling, T.

PY - 1995

Y1 - 1995

N2 - ENOD12 is one of the first nodulin genes expressed upon inoculation with Rhizobium and also purified Nod factors are able to induce ENOD12 expression. The ENOD12 gene family in pea (Pisum sativum) has two members. A cDNA clone representing PsENOD12A [26] and a PsENOD12B genomic clone [7] have been previously described. The isolation and characterization of a PsENOD12A genomic clone is presented in this paper. By using a Vicia hirsuta-Agrobacterium rhizogenes transformation system it is shown that both genes have a similar expression pattern in transgenic V. hirsuta root nodules. Promoter analyses of both PsENOD12 promoters showed that the 200 bp immediately upstream of the transcription start are sufficient to direct nodule-specific and Nod factor-induced gene expression.

AB - ENOD12 is one of the first nodulin genes expressed upon inoculation with Rhizobium and also purified Nod factors are able to induce ENOD12 expression. The ENOD12 gene family in pea (Pisum sativum) has two members. A cDNA clone representing PsENOD12A [26] and a PsENOD12B genomic clone [7] have been previously described. The isolation and characterization of a PsENOD12A genomic clone is presented in this paper. By using a Vicia hirsuta-Agrobacterium rhizogenes transformation system it is shown that both genes have a similar expression pattern in transgenic V. hirsuta root nodules. Promoter analyses of both PsENOD12 promoters showed that the 200 bp immediately upstream of the transcription start are sufficient to direct nodule-specific and Nod factor-induced gene expression.

KW - ENOD12

KW - gene regulation

KW - hairy roots

KW - Rhizobium

KW - transgenic root nodules

KW - Vicia hirsuta

U2 - 10.1007/BF00032670

DO - 10.1007/BF00032670

M3 - Article

SN - 0167-4412

VL - 28

SP - 1103

EP - 1110

JO - Plant Molecular Biology

JF - Plant Molecular Biology

ER -

A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression.

Abstract

Keywords

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