3C in maize and arabidopsis

Blaise Weber, Suraj Jamge, Maike Stam*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

4 Citations (Scopus)

Abstract

With Chromosome Conformation Capture (3C), the relative interaction frequency of one chromosomal fragment with another can be determined. The technique is especially suited for unraveling the 3D organization of specific loci when focusing on aspects such as enhancer–promoter interactions or other topological conformations of the genome. 3C has been extensively used in animal systems, among others providing insight into gene regulation by distant cis-regulatory elements. In recent years, the 3C technique has been applied in plant research. However, the complexity of plant tissues prevents direct application of existing protocols from animals. Here, we describe an adapted protocol suitable for plant tissues, especially Arabidopsis thaliana and Zea mays.
Original languageEnglish
Title of host publicationPlant Chromatin Dynamics
Subtitle of host publicationMethods and Protocols
EditorsM. Bemer, C. Baroux
PublisherHumana Press Inc.
Pages247-270
ISBN (Electronic)9781493973187
ISBN (Print)9781493973170
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1675
ISSN (Print)1064-3745

Keywords

  • 3C protocol
  • Arabidopsis thaliana
  • Chromosome Conformation Capture
  • Plant
  • Zea mays

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