14-3-3 proteins interact with a 13-lipoxygenase, but not with a 9-lipoxygenase

Wessel L. Holtman*, Michael R. Roberts, Berry J. Oppedijk, Christa Testerink, Mieke J. Van Zeijl, Mei Wang

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalFEBS Letters
Volume474
Issue number1
DOIs
Publication statusPublished - 26 May 2000
Externally publishedYes

Keywords

  • 14-3-3 protein
  • Interaction
  • Lipoxygenase

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