TY - JOUR
T1 - 136 Increased pre-weaning metabolizable energy supply affects the expression of genes related to adipogenesis and lipid metabolism in the skeletal muscle of prepubertal Holstein heifers.
AU - Hare, K.
AU - Romao, J.
AU - Leal, L.
AU - Hooiveld, G.
AU - Soberon, F.
AU - Berends, H.
AU - Boekschoten, M.
AU - van Amburgh, M.
AU - Martín-Tereso, J.
AU - Steele, M.
PY - 2018/12/7
Y1 - 2018/12/7
N2 - It is unknown how dairy heifer skeletal muscle responds to increased pre-weaning ME intake. Therefore, the objective was to determine how elevated ME provision during the pre-weaning period affects the expression of the skeletal muscle transcriptome. Holstein heifers consumed either 5.9 (elevated, ELE, n = 4) or 2.8 ± 0.2 Mcal ME (control, n = 6) per day in milk replacer (28% CP, 15% fat). Heifers were harvested at d 54 and semimembranosus muscle was snap-frozen in liquid N2 and stored at -80°C until analysis with Affymetrix Bovine Gene 1.1 ST arrays to profile transcriptome expression. A moderated t-test using a Bayesian model was used to generate the gene data set. Ingenuity Pathway Analysis software analyzed the fold change (FC; ELE/control) in expression of 18,811 genes, of which 577 met the threshold (P < 0.01) for analysis. These results reflect myocyte and intramuscular adipocyte contributions to the transcriptome expression. Decreased or increased activity was declared using a z-score ≤ -2 or ≥ 2, respectively, and trends were discussed when -2 < z < 0 or 0 < z < 2. PPARGC1A was identified as the top affected (P < 0.001, 22 molecules) upstream regulator within the transcriptome. Based on FC of gene expression, triacylglycerol concentration was predicted to increase (z = 2.043, P < 0.001, 26 molecules affected) while the quantity of lipid peroxide may decrease (z = -1.982, P = 0.003) for ELE calves. As a result, lipid metabolism was the most affected (P ≤ 0.017, 96 molecules) cellular function, while the adipogenic pathway was determined to be the third top affected (P = 0.010, 118 molecules) canonical pathway. Gene results suggest that consumption of increased ME during the pre-weaning period affected adipogenesis and increased triacylglycerol storage while simultaneously reducing lipid peroxidation within skeletal muscle.
AB - It is unknown how dairy heifer skeletal muscle responds to increased pre-weaning ME intake. Therefore, the objective was to determine how elevated ME provision during the pre-weaning period affects the expression of the skeletal muscle transcriptome. Holstein heifers consumed either 5.9 (elevated, ELE, n = 4) or 2.8 ± 0.2 Mcal ME (control, n = 6) per day in milk replacer (28% CP, 15% fat). Heifers were harvested at d 54 and semimembranosus muscle was snap-frozen in liquid N2 and stored at -80°C until analysis with Affymetrix Bovine Gene 1.1 ST arrays to profile transcriptome expression. A moderated t-test using a Bayesian model was used to generate the gene data set. Ingenuity Pathway Analysis software analyzed the fold change (FC; ELE/control) in expression of 18,811 genes, of which 577 met the threshold (P < 0.01) for analysis. These results reflect myocyte and intramuscular adipocyte contributions to the transcriptome expression. Decreased or increased activity was declared using a z-score ≤ -2 or ≥ 2, respectively, and trends were discussed when -2 < z < 0 or 0 < z < 2. PPARGC1A was identified as the top affected (P < 0.001, 22 molecules) upstream regulator within the transcriptome. Based on FC of gene expression, triacylglycerol concentration was predicted to increase (z = 2.043, P < 0.001, 26 molecules affected) while the quantity of lipid peroxide may decrease (z = -1.982, P = 0.003) for ELE calves. As a result, lipid metabolism was the most affected (P ≤ 0.017, 96 molecules) cellular function, while the adipogenic pathway was determined to be the third top affected (P = 0.010, 118 molecules) canonical pathway. Gene results suggest that consumption of increased ME during the pre-weaning period affected adipogenesis and increased triacylglycerol storage while simultaneously reducing lipid peroxidation within skeletal muscle.
U2 - 10.1093/jas/sky404.767
DO - 10.1093/jas/sky404.767
M3 - Article
SN - 0021-8812
VL - 96
SP - 348
EP - 349
JO - Journal of Animal Science
JF - Journal of Animal Science
IS - suppl_3
ER -