1-Octen-3-ol inhibits conidia germination of Penicillium paneum despite of mild effects on membrane permeability, respiration, intracellular pH, and changes the protein composition

G.S. Chitarra, T. Abee, F.M. Rombouts, J. Dijksterhuis

Research output: Contribution to journalArticleAcademicpeer-review

63 Citations (Scopus)

Abstract

1-Octen-3-ol is a volatile germination self-inhibitor produced by Penicillium paneum that blocks the germination process. The size of conidia treated with 1-octen-3-ol was similar to that of freshly harvested conidia. Exposure to 1-octen-3-ol resulted in staining of 10¿20% of the conidia with PI and TOTO, fluorescent DNA probes that cannot enter cells with an intact membrane, whereas only 3¿5% of non-treated conidia were stained. Addition of 1-octen-3-ol to germinating conidia resulted in transient dissipation of the pH gradient. From this, we conclude that slight permeabilisation of the fungal membrane occurs in the presence of the inhibitor. Two-dimensional gel electrophoresis analysis of protein patterns revealed striking differences between non-germinated conidia, germinated conidia and 1-octen-3-ol-treated conidia. In conclusion, 1-octen-3-ol has mild effects on the plasma membrane, but interferes with essential metabolic processes, such as swelling and germination of the conidia, but in a reversible manner
Original languageEnglish
Pages (from-to)67-75
JournalFEMS microbiology ecology
Volume54
Issue number1
DOIs
Publication statusPublished - 2005

Keywords

  • agaricus-bisporus
  • 10-oxo-trans-8-decenoic acid
  • listeria-monocytogenes
  • pleurotus-ostreatus
  • fluorescent-probes
  • self-inhibitor
  • metabolism
  • cells
  • indicators
  • ascospores

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