Untargeted and semi-targeted methods for detection of foodborne pathogens

Testing of food is a crucial tool for mitigation of microbiological risks, both in verification of food safety measures and in response to foodborne outbreaks. At present, culture-based detection methods for foodborne pathogens are the gold standard. However, these protocols are lengthy and labor-intensive, resulting in long times-to-results. The application of DNA sequencing, and in particular of metagenomics, can establish a faster detection of multiple pathogens in food, together with providing additional useful information to risk management. In this project, two different, but potentially compatible, strategies for the application of metagenomics to pathogen detection in food will be considered, and research will be performed towards their applicability.