Un-RiPP: A high-throughput approach for discovering ribosomal-origin antimicrobials.

Antimicrobial resistance is a major global problem and exhaustion of traditional sources of antimicrobials has slowed the race to discover new drugs, emphasizing the need for innovative strategies to develop and validate new antimicrobials. Here I aim to test a newly developed innovative approach to identify and characterize new antimicrobials from microbiomes, specifically those focusing on lanthipeptides class-l from the largely untapped natural product-encoding gene clusters in bacterial genomes. One of the main challenges in developing lanthipeptides class-l is their production in microbial hosts. My approach addresses this by developing a robust system for heterologous production thereof in microbes.