Ontwikkeling methoden Next Generation Sequencing toepassingen (KB-21-002-006)

Next generation sequencing of pathogens has gained a prominent role within the CVI. Most of these pathogens are derived from field samples containing many other viruses and bacteria but usually also contain a significant proportion of host-derived nucleic acids (DNA/RNA). Much time and money is usually consumed to find the right culturing conditions of for instance viruses on host cells. Due to the time constraints during for instance outbreak management it is desirable to be able to rapidly determine the genome sequence of viral pathogen as directly from the original sample as possible. At the moment our current nucleic acid isolation procedures are not suitable for direct full genome sequencing of the causative viral pathogens directly from for instance sera of fecal samples. A strong enrichment of pathogen-specific nucleic acids is required in which host-derived nucleic acids are depleted efficiently. Several techniques will be used to enrich DNA/RNA of the causal pathogen from the other non-pathogens and contaminating host-background.