Omics Biomarkers (KB-17-003.02-005)

  • Smits, Mari (Project Leader)

Project: LVVN project

Project Details

Description

In this project we will develop a combination of technologies for detection, analysis, and use of biomarkers in milk, aiming at early detection of pregnancy of cows. The reason for this choice is that there is a market demand from the industry for a milk test for early detection of pregnancy, and this project will be performed together with, and with additional funding from, two Dutch companies: QLIP and CRV. CRV breeds high-productive dairy cattle and  QLIP performs  routine milk testings.

 

In this project we will develop new methods and technologies for 1) detection of biomarkers, 2) proteome-wide data analysis to find associations, and 3) multiplex measurement of biomarkers in practical applications. This project  aims for improved resolution of the milk proteome, using Fourier Transform Mass Spectrometry (FTMS) in conjunction with an optimized protocol for sample preparation. FTMS is very sensitive and offers the possibility of  identification of potential biomarkers. Second, a systems-biology approach of proteome-wide  modeling of expression levels in relation to a trait of interest will be developed, to combine many small signals that in combination can have a strong association with the trait or state of interest. Third, we will also determine mid-infrared (MIR) profiles of the milk samples, and analyze the MIR data in conjunction with the proteome data. 

 

Output of this project will be 1) Improved methods for detection of the milk proteome. 2) Improved and validated method for proteome-wide modeling of expression levels in relation to a state or trait. 3) Exploration of application of developed technologies to other traits relevant to agroproduction. 4) Publications, 5) Contributions to scientific symposia, and KennisOnline

The market for the pregnancy test and other milk biomarker tests is (1) The Dutch (and world-wide) dairy production sector, (2) The broader livestock production sector.

StatusFinished
Effective start/end date1/01/1131/12/14

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