The rapid development in DNA sequencing techniques facilitate an alternative and cost efficient approach for biodiversity assessment and make an early detection of invasive species possible. In this technique, named 'metabarcoding', universal primers are used in order to identify a large number of species belonging to several species groups. However, the identification success differs among species groups, and species within several species groups are not detectable at all. In this study the development of primer sets is proposed in order to detect yet not identifiable species in North Sea and Wadden Sea and to provide an overview of identification success per primer and species group to facilitate primer choice.
|Effective start/end date||1/01/17 → 31/12/17|