LWV20232 Identifying and characterizing Verticillium resistance in potato (BO-68-001-035)

So far, Verticillium wilt is being controlled by environmentally harmful soil fumigants. In this project, we aim to identify and characterize genetic resistance against Verticillium in wild Solanum accessions. We will exploit a functional effectoromics breeding strategy, combined with modern sequencing technologies. By expressing proteins secreted by the pathogen (effectors), we will detect the corresponding resistance genes in Solanum plants based on specific defence responses in functional assays. With the available Verticillium genome sequence, knowledge on effectors and in house expertise on sequencing strategies such as resistance gene enrichment sequencing and derivatives thereof, the isolation of new resistance genes from these sources is straightforward. In parallel to the effector assays, we will subject wild Solanum plants to diseases trials and optimize a diagnostic assay for Verticillium. This effector-assisted breeding project will lead to the identification of resistant sources, molecular markers and resistance genes against Verticillium in potato. These results will enable the involved companies to breed for novel varieties that are resistant to Verticillium wilt.