Incorporating upstream process history and strain degeneration into metabolic modelling of production strains in prolonged fed-batch fermentations for identifying strain development targets

Project: PhD

Project Details


Continuous or extended cultivation in industrial biotechnological processes is considered one of the holy grails in process optimisation that could greatly increase profitability. Strain degeneration is one of the main obstacles in achieving prolonged cultivation. Although there are some hypotheses concerning adaptation to heterogeneities of scaled-up processes, genetic instability and subpopulation formation within a reactor, no conclusive answer has been found. Remarkably, research into large scale cultivation processes does not take the upstream seed train with its process heterogeneities and cultivation time into account. Furthermore, the cumulative effect of adaptations to process heterogeneities, stress responses, genetic instability and their subsequent effects on sub populations have not been connected to process models before. The proposed research aims to develop this model capability to be able to predict strain degeneration onset, optimise industrial processes to prolong cultivation and identify strain development targets to make strain more robust for industrial use.
Effective start/end date1/09/23 → …


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