The Adverse Outcome Pathway (AOP) concept is a new way of thinking within the toxicology and chemical risk assessment and is considered to be the basis for new mechanism-based toxicity testing and a framework for the development of alternative methods for animal experimentation. This project aims to construct a panel of fluorescence-based bioassays representing different cellular processes that upon perturbation can result in toxicity. The fluorescence is measured through (real-time) cell imaging. Various fluorophores can be applied allowing to study the effects of compounds on different processes and pathways at the same time. The assays, together with a cell imager, will be used for hazard identification of (new) compounds, in particular those related to food.
|Effective start/end date||1/01/13 → 31/12/19|