Exploiting native endowments by re-factoring, re-programming and implementing novel control loops in Pseudomonas putida for bespoke biocatalysis

    Project: EU research project

    Project Details

    Description

    We aim to engineer the lifestyle of Pseudomonas putida to generate a tailored, re-factored chassis with highly attractive new-to-nature properties, thereby opening the door to the production of thus far non-accessible compounds. This industrially driven project capitalises on the outstanding metabolic endowment and stress tolerance capabilities of this versatile bacterium for the production of specialty and bulk chemicals. Specifically, we will build streamlined P. putida strains with improved ATP availability utilizing this power on demand, decoupled from growth. The well-characterized, streamlined and re-factored strain platform will offer easy-to-use plug-in opportunities for novel, DNA-encoded functions under the control of orthogonal regulatory systems. To this end, we will deploy a concerted approach of genome refactoring, model-driven circuit design, implementation of ATP control loops, structured modelling and metabolic engineering. By drawing on a starkly improved, growth-uncoupled ATP-biosynthetic machinery, empowered P. putida strains will be able to produce a) n-butanol and isobutanol and their challenging gaseous derivatives 1-butene (BE) and (iso-)butadiene (BDE) using a novel, new-to-nature route starting from glucose, as well as b) new active ingredients for crop protection, such as tabtoxin, a high-value, ß-lactam-based secondary metabolite with a huge potential as a new herbicide. The game-changing innovations brought in – in particular the uncoupling of ATP-synthesis and production from growth - will provide strong versatility, enhanced efficiency and efficacy to the production processes, thereby overcoming current bottlenecks, matching market needs and fostering high-level research growth and development.
    AcronymEmPowerPutida
    StatusFinished
    Effective start/end date1/05/1530/04/19

    Research Output

    • 5 Article
    • 1 Abstract
    • 1 Comment/Letter to the editor

    An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida

    Batianis, C., Kozaeva, E., Damalas, S., Martin Pascual, M., Volke, D., Nikel, P. I. & Martins dos Santos, V. A. P., Mar 2020, In : Microbial Biotechnology. 13, 2, p. 368-385

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
  • 2 Citations (Scopus)

    Consistency, inconsistency, and ambiguity of metabolite names in biochemical databases used for genome-scale metabolic modelling

    Pham, N., van Heck, R. G. A., van Dam, J. C. J., Schaap, P. J., Saccenti, E. & Suarez-Diez, M., 6 Feb 2019, In : Metabolites. 9, 2, 28.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
  • 4 Citations (Scopus)

    Pseudomonas putida KT2440 is HV1 certified, not GRAS

    Kampers, L. F. C., Volkers, R. J. M. & Martins dos Santos, V. A. P., Sep 2019, In : Microbial Biotechnology. 12, 5, p. 845-848

    Research output: Contribution to journalComment/Letter to the editorAcademic

    Open Access
  • 2 Citations (Scopus)