Engineering of Mycoplasma pneumoniae as a broad-spectrum animal vaccine

    Project: EU research project

    Project Details

    Description

    Mycoplasmas are the smallest cell wall less, free-living microorganisms. The lack of a cell wall makes them resistant to many of the common antibiotics. Every year, infections caused by Mycoplasmas in poultry, cows, and pigs, result in multimillion euros losses in USA and Europe. Currently, there are vaccines against M hyopneumoniae in pigs and M gallisepticum and M synoviae in poultry. However, there is no vaccination against many Mycoplasma species infecting pets, humans and farm animals (ie M bovis cow infection). Mycoplasma species in many cases are difficult to grown in axenic culture and those that grow need a complex media with animal serum. In large scale production of Mycoplasma species for vaccination aside from the high cost of animal serum, more important is the high irreproducibility in the production process and the possible contamination with animal viruses. All this together highlights what European industry needs:i) a defined cheap reproducible medium that is animal serum free and ii) an universal Mycoplasma chassis that could be used in a pipeline to vaccinate against Mycoplasma species, as well as any pathogen. M pneumoniae is an ideal starting point for designing such a vaccine chassis. It has a small genome (860 kb) and it is probably the organism with the most comprehensive systems biology data acquired so far. By genome comparison, metabolic modeling and rationally engineering its genome, we will create a vaccine chassis that will be introduced into an industrial pipeline. The process will be guided by the second world largest industry on animal vaccination (MSD), as well as a SME specialized on peptide display and screening. This will ensure the exploitation and commercialization of our work contributing to maintain Europe privileged position in this field. Our ultimate goal is to meet the needs of the livestock industry,taking care of ethical issues, foreseeable risks, and prepare effective dissemination and training material for the public.
    AcronymMycoSynVac
    StatusFinished
    Effective start/end date1/04/1531/03/20

    Research Output

    Open Access
  • Basic Phytochrome B Calculations

    Smith, R. W. & Fleck, C., 18 Jul 2019, Phytochromes: Methods and Protocols. Hiltbrunner, A. (ed.). Humana Press Inc., p. 121-133 13 p. (Methods in Molecular Biology; vol. 2026).

    Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

  • Phytochrome B dynamics departs from photoequilibrium in the field

    Sellaro, R., Smith, R. W., Legris, M., Fleck, C. & Casal, J. J., Feb 2019, In : Plant Cell and Environment. 42, 2, p. 606-617

    Research output: Contribution to journalArticleAcademicpeer-review

  • 6 Citations (Scopus)