Determining ROS-dependent ligands for Cysteine-Rich Receptors

Project: PhD

Project Details


Extracellular produced Reactive Oxygen Species (ROS) are important signaling molecules in plants, but their perception is poorly understood. We propose that fluctuating ROS levels are sensed by Cysteine Rich Protein (CRP) complexes, through oxidative modifications on cysteine residues. Members of the CRPs in Arabidopsis are Cysteine-Rich Receptor-Like Kinases (CRKs), Plasmodesmata Localized Proteins (PDLPs) and Cysteine-Rich Repeat Secretory Proteins (CRRSPs), characterized by the presence of two Domains of Unknown Function 26 (DUF26) in their extracellular domain. Canonical receptor activation often requires interaction with its ligand. Since Arabidopsis thaliana is secreting over a thousand small secreted peptides (SPPs) during developmental processes and biotic and abiotic stresses, we hypothesize that SPPs could serve as ligands in CRPs complexes. We aim to assign peptide ligands to CRKs with a high-density peptide array and characterize CRRSPs-CRKs-PDLPs interactions in fluctuating ROS conditions.
Effective start/end date1/04/22 → …


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