‘CARFing’ out the signalling network of CRISPR-Cas

This project will have both a fundamental and applied aspects. For the fundamental aspect, we aim to characterize predicted CARF and SAVED proteins, their enzymatic activities, and how these contribute to immune defense. Following this, we plan to repurpose these proteins for different applications, such as in diagnostics or gene editing, taking advantage of the tight control of type III CRISPR-Cas systems over their activities.