Activities per year
Project Details
Description
Reverse genetics for African horsesickness virus (AHSV) was developed. The method was subsequently used to exchange the serotype-determining genome segment for all nine serotypes in well-producible AHSV. The method was also used to knock-out the NS3-expression. A serology test detecting antibodies against this immunogenic protein is currently under development by others. On this way, two sets of viruses were generated for all nine serotypes. These can be used for production of dead AHS vaccines and for the production of live-attenuated vaccines, since the ancestor virus has been applied in both ways during the Spanish AHS outbreak.
In addition, a lot of knowledge as generated by research on the related Bluetongue virus was used. Similar to BT DISA vaccine for Bluetongue (DISA: Disabled Infectious Single Animal: patent application 16 may 2013), AHSV variants without NS3 expression are very promising candidates for the development of AHS DISA vaccines.
Description problem/question
Vaccine for AHS is not available
Research objectives
Vaccine development for African horse sickness
Results and products
Exchange method for AHSV
Production viruses for six serotypes of AHSV
AHS-vaccine candidates based on inactivated AHSV
Status | Finished |
---|---|
Effective start/end date | 1/01/11 → 31/12/14 |
LVVN programmes
- Beleidsondersteunend onderzoek (BO)
Fingerprint
Activities
- 3 Lecture/seminar/webinar
-
Progressie bij de vaccinontwikkeling voor Afrikaanse paardenpest. Een calamiteit minder?
van Rijn, P. (Speaker)
19 Dec 2014 → …Activity: Talk/presentation/lecture › Lecture/seminar/webinar › Other
-
Development of reverse genetics for African horse sickness virus and its use to rescue reassortants and mutants.
van Rijn, P. (Speaker)
1 Oct 2013 → …Activity: Talk/presentation/lecture › Lecture/seminar/webinar › Other
-
Reverse genetics for African horse sickness virus; Development of rationally designed vaccines
van de Water, S. (Speaker)
2 Dec 2013 → …Activity: Talk/presentation/lecture › Lecture/seminar/webinar › Other