AF-EU-18023 - LOGIC (BO-64-101-003, BO-46-001-008)

The objective of this project is to develop a prototype kit for multi-analyte antibiotic screening in several food matrices that is suitable for use by the food industry. This project will provide a novel and unique rapid multiplex test which is not currently offered on the market anywhere worldwide. The ambition of this project is to provide a prototype that will have a time to results of less than 10mins/sample with detection limits lower than what is required to meet legislation globally.

An array of binding ligands (carrier proteins or antibodies) will be printed on a suitable solid binding surface by ultra-low volume dispensing. Applying printed spots of binding ligands enables the very efficient use of these ligands (droplets of approximately 1 nL of sample) and allows for a multiplicity of samples to be detected on a very limited area of solid binding surface. Nevertheless, running the test is as simple as an over-the-counter pregnancy hormone test kit. Such rapid multiplex assays are not in the market due to several challenges that will be addressed in this project: the lack of large-scale production possibilities for these multiplex tests, the lack of cutting equipment suited for cutting in between arrays and the lack of a reader or smartphone application to read the results of such a test.