Johannes Hohlbein

dr.

    20122020
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    Research Output 2013 2020

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    2020

    Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

    Vink, J. N. A., Martens, K. J. A., Vlot, M., McKenzie, R. E., Almendros, C., Estrada Bonilla, B., Brocken, D. J. W., Hohlbein, J. & Brouns, S. J. J., 2 Jan 2020, In : Molecular Cell. 77, 1, p. 39-50.e10

    Research output: Contribution to journalArticleAcademicpeer-review

    Clustered Regularly Interspaced Short Palindromic Repeats
    Interspersed Repetitive Sequences
    Bacteria
    DNA
    RNA
    2019
    1 Citation (Scopus)

    Evaluating single-particle tracking by photo-activation localization microscopy (sptPALM) in Lactococcus lactis

    van Beljouw, S. P. B., van der Els, S., Martens, K. J. A., Kleerebezem, M., Bron, P. A. & Hohlbein, J., 8 Mar 2019, In : Physical Biology. 16, 3, 1 p.

    Research output: Contribution to journalArticleAcademicpeer-review

    Lactococcus lactis
    Microscopy
    CRISPR-Cas Systems
    Fermentation
    Lactic Acid
    2 Citations (Scopus)

    High-throughput, non-equilibrium studies of single biomolecules using glass-made nanofluidic devices

    Fontana, M., Fijen, C., Lemay, S. G., Mathwig, K. & Hohlbein, J., 7 Jan 2019, In : Lab on a Chip. 19, 1, p. 79-86

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
    Nanofluidics
    Biomolecules
    Glass
    DNA
    Throughput

    Substrate conformational dynamics facilitate structure-specific recognition of gapped DNA by DNA polymerase

    Craggs, T. D., Sustarsic, M., Plochowietz, A., Mosayebi, M., Kaju, H., Cuthbert, A., Hohlbein, J., Domicevica, L., Biggin, P. C., Doye, J. P. K. & Kapanidis, A. N., 18 Nov 2019, In : Nucleic acids research. 47, 20, p. 10788-10800 13 p., gkz797.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
    DNA-Directed DNA Polymerase
    DNA
    DNA Polymerase I
    Nucleic Acid Conformation
    DNA Primers
    2 Citations (Scopus)

    Visualisation of dCas9 target search in vivo using an open-microscopy framework

    Martens, K. J. A., van Beljouw, S. P. B., van der Els, S., Vink, J. N. A., Baas, S., Vogelaar, G. A., Brouns, S. J. J., van Baarlen, P., Kleerebezem, M. & Hohlbein, J., 7 Aug 2019, In : Nature Communications. 10, 1, 3552.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
    Clustered Regularly Interspaced Short Palindromic Repeats
    Microscopy
    Microscopic examination
    Screening
    Plasmids
    2018
    8 Citations (Scopus)
    Open Access
    Program processors
    Microscopic examination
    microscopy
    Molecules
    molecules
    40 Citations (Scopus)

    Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

    Hellenkamp, B., Schmid, S., Doroshenko, O., Opanasyuk, O., Kühnemuth, R., Rezaei Adariani, S., Ambrose, B., Aznauryan, M., Barth, A., Birkedal, V., Bowen, M. E., Chen, H., Cordes, T., Eilert, T., Fijen, C., Gebhardt, C., Götz, M., Gouridis, G., Gratton, E., Ha, T. & 43 others, Hao, P., Hanke, C. A., Hartmann, A., Hendrix, J., Hildebrandt, L. L., Hirschfeld, V., Hohlbein, J., Hua, B., Hübner, C. G., Kallis, E., Kapanidis, A. N., Kim, J. Y., Krainer, G., Lamb, D. C., Lee, N. K., Lemke, E. A., Levesque, B., Levitus, M., McCann, J. J., Naredi-Rainer, N., Nettels, D., Ngo, T., Qiu, R., Robb, N. C., Röcker, C., Sanabria, H., Schlierf, M., Schröder, T., Schuler, B., Seidel, H., Streit, L., Thurn, J., Tinnefeld, P., Tyagi, S., Vandenberk, N., Vera, A. M., Weninger, K. R., Wünsch, B., Yanez-Orozco, I. S., Michaelis, J., Seidel, C. A. M., Craggs, T. D. & Hugel, T., 31 Aug 2018, In : Nature Methods. 15, 9, p. 669-676

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
    Benchmarking
    Energy Transfer
    Energy transfer
    Molecules
    Structural Models
    2017

    A Nanofluidic Mixing Device for High-throughput Fluorescence Sensing of Single Molecules

    Mathwig, K., Fijen, C., Fontana, M., Lemay, S. G. & Hohlbein, J. C., 2017, In : Procedia Technology. 27, p. 141-142 2 p.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
    Nanofluidics
    Fluorescence
    Throughput
    Molecules
    Energy transfer
    9 Citations (Scopus)

    A single-molecule FRET sensor for monitoring DNA synthesis in real time

    Fijen, C., Monton Silva, A., Hochkoeppler, A. & Hohlbein, J. C., 2017, In : Physical Chemistry Chemical Physics. 19, 6, p. 4222-4230

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
    Energy transfer
    deoxyribonucleic acid
    energy transfer
    Molecules
    DNA-Directed DNA Polymerase
    12 Citations (Scopus)

    Visualization of BRI1 and SERK3/BAK1 nanoclusters in Arabidopsis roots

    Hutten, S. J., Hamers, D. S., Den Toorn, M. A., Van Esse, W., Nolles, A., Bücherl, C. A., De Vries, S. C., Hohlbein, J. & Borst, J., 2017, In : PLoS ONE. 12, 1, e0169905.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
    Brassinosteroids
    brassinosteroids
    Nanoclusters
    somatic embryogenesis
    Arabidopsis
    2016
    20 Citations (Scopus)
    Open Access
    Energy transfer
    Fluorescence
    energy transfer
    proteins
    Proteins
    22 Citations (Scopus)

    Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers

    Ploetz, E., Lerner, E., Husada, F., Roelfs, M., Chung, S., Hohlbein, J., Weiss, S. & Cordes, T., 2016, In : Scientific Reports. 6, 33257.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
    Energy transfer
    Fluorescence
    Proteins
    Assays
    Laser excitation
    7 Citations (Scopus)

    New technologies for DNA analysis – a review of the READNA Project

    McGinn, S., Bauer, D., Brefort, T., Dong, L., El-Sagheer, A., Elsharawy, A., Evans, G., Falk-Sörqvist, E., Forster, M., Fredriksson, S., Freeman, P., Freitag, C., Fritzsche, J., Gibson, S., Gullberg, M., Gut, M., Heath, S., Heath-Brun, I., Heron, A. J., Hohlbein, J. & 47 others, Ke, R., Lancaster, O., Le Reste, L., Maglia, G., Marie, R., Mauger, F., Mertes, F., Mignardi, M., Moens, L., Oostmeijer, J., Out, R., Pedersen, J. N., Persson, F., Picaud, V., Rotem, D., Schracke, N., Sengenes, J., Stähler, P. F., Stade, B., Stoddart, D., Teng, X., Veal, C. D., Zahra, N., Bayley, H., Beier, M., Brown, T., Dekker, C., Ekström, B., Flyvbjerg, H., Franke, A., Guenther, S., Kapanidis, A. N., Kaye, J., Kristensen, A., Lehrach, H., Mangion, J., Sauer, S., Schyns, E., Tost, J., van Helvoort, J. M. L. M., van der Zaag, P. J., Tegenfeldt, J. O., Brookes, A. J., Mir, K., Nilsson, M., Willcocks, J. P. & Gut, I. G., 2016, In : New Biotechnology. 33, 3, p. 311-330

    Research output: Contribution to journalArticleAcademicpeer-review

    Nucleic acids
    Nucleic Acids
    DNA
    Technology
    Equipment and Supplies
    4 Citations (Scopus)

    Single Molecule 3D Orientation in Time and Space: A 6D Dynamic Study on Fluorescently Labeled Lipid Membranes

    Börner, R., Ehrlich, N., Hohlbein, J. & Hübner, C. G., 2016, In : Journal of Fluorescence. 26, 3, p. 963-975

    Research output: Contribution to journalArticleAcademicpeer-review

    Membrane Lipids
    Fluorescence
    Fluorescent Dyes
    Molecules
    Coloring Agents
    2015
    17 Citations (Scopus)

    Camera-based single-molecule FRET detection with improved time resolution

    Farooq, S. & Hohlbein, J. C., 2015, In : Physical Chemistry Chemical Physics. 17, p. 27862-27872

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
    Laser excitation
    Cameras
    cameras
    Molecules
    excitation
    6 Citations (Scopus)

    Complex Coacervate Core Micelles with Spectroscopic Labels for Diffusometric Probing of Biopolymer Networks

    Bourouina, N., De Kort, D. W., Hoeben, F. J. M., Janssen, H. M., Van As, H., Hohlbein, J., Van Duynhoven, J. P. M. & Kleijn, J. M., 2015, In : Langmuir. 31, 46, p. 12635-12643

    Research output: Contribution to journalArticleAcademicpeer-review

    Biopolymers
    biopolymers
    Micelles
    Labels
    micelles
    2014
    73 Citations (Scopus)

    Alternating-laser excitation: single-molecule FRET and beyond

    Hohlbein, J. C., Craggs, T. D. & Cordes, T., 2014, In : Chemical Society Reviews. 43, p. 1156-1171

    Research output: Contribution to journalArticleAcademicpeer-review

    Laser excitation
    Molecules
    Energy transfer
    Fluorophores
    Biological systems
    8 Citations (Scopus)

    Studying DNA-protein interactions with single-molecule Förster resonance energy transfer

    Farooq, S., Fijen, C. & Hohlbein, J. C., 2014, In : Protoplasma. 251, 2, p. 317-332

    Research output: Contribution to journalArticleAcademicpeer-review

    Energy Transfer
    energy transfer
    DNA
    Proteins
    proteins
    2013
    44 Citations (Scopus)

    Conformational landscapes of DNA polymerase I and mutator derivates establish fidelity checkpoints for nucleotide insertion

    Hohlbein, J. C., Aigrain, L., Craggs, T. D., Bermek, O., Potapova, O., Shoolizadeh, P., Grindley, N. D. F., Joyce, C. M. & Kapanidis, A. N., 2013, In : Nature Communications. 4, 11 p., 2131.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
    DNA Polymerase I
    nucleotides
    Conformations
    insertion
    Nucleotides