Transgenic plant analysis using NGS



Both Transgenic and control Arabidopsis thaliana plants were analysed by whole genome resequencing. Transformation events were analysed in detail showing multiple T-DNA insertions, resulting in deletions, insertions, SNPs, rearrangements and partial insertions.
Date made available1 Jan 2017
PublisherWageningen UR

Research Output

Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere

Schouten, H. J., van de Geest, H., Papadimitriou, S., Bemer, M., Schaart, J. G., Smulders, R., Perez, G. S. & Schijlen, E., Mar 2017, In : Plant Cell Reports. 36, 3, p. 493-504

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
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    Schouten, H. J. (Creator), van de Geest, H. C. (Creator), Papadimitriou, S. (Creator), Bemer, M. (Creator), Schaart, J. G. (Creator), Smulders, M. J. M. (Creator), Sanchez Perez, G. F. (Creator), Schijlen, E. G. W. M. (Creator) (1 Jan 2017). Transgenic plant analysis using NGS. Wageningen UR.