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Bacteria are able to cope with the challenges of sudden increase of salinity by activating adaptation mechanisms. In this study, exponentially growing cells of the food-borne pathogen Bacillus cereus ATCC 14579 were exposed to both mild (2.5% NaCl w/v) and severe (5% NaCl w/v) salt stress conditions. B. cereus continued growth at a reduced rate when shifted to mild salt stress. Exposure to severe salt stress resulted in a lag period, and after 60 min cellular growth was resumed filamentously. Whole-genome expression analyses of cells exposed to 2.5% salt stress revealed an overlap with that of cells exposed to 5% salt stress, suggesting that the corresponding genes (n = 147) were involved in a general salt stress response. Up-regulation of osmolyte, Na+/H+ and di-/tripeptide transporters and activation of an oxidative stress response were important aspects of this general salt stress response. Activation of this response may confer cross-protection towards other stresses, and increased resistance to heat and H2O2 was indeed observed. Notably, a temporal shift was observed between the observed transcriptome and phenotype responses of severely salt-stressed cells including cellular filamentation, reduced chemotaxis performance, catalase activity and optimal oxidative stress resistance. The linkage of transcriptomes and phenotypic characteristics can contribute to a better understanding of cellular stress adaptation strategies and possible cross protection mechanisms.
|Date made available||22 Jun 2009|
Phenotypic and Transcriptomic Analyses of Mildly and Severely Salt-Stressed Bacillus cereus ATCC 14579 Cellsden Besten, H. M. W., Mols, J. M., Moezelaar, R., Zwietering, M. H. & Abee, T., 2009, In : Applied and Environmental Microbiology. 75, 12, p. 4111-4119
Research output: Contribution to journal › Article › Academic › peer-review
den Besten, H. (Creator), Mols, J. M. (Creator), Moezelaar, R. (Creator), Zwietering, M. (Creator), Abee, T. (Creator) (22 Jun 2009). Transcriptome analyses of 2.5% and 5% NaCl-stressed B. cereus ATCC 14579. Wageningen University.