Transcriptional profiles between mp mutant seedlings and transgenics carrying the dexamethasone-inducible GR-bdl protein

  • Alexandra Schlereth (Creator)
  • Gerd Juergens (Creator)
  • Dolf Weijers (Creator)

Dataset

Description

In order to identify targets of the transcription factor AUXIN RESPONSE FACTOR5 / MONOPTEROS (ARF5/MP), we compared transcriptomes of mp-B4149 mutant seedlings (9 day-old) and seedlings carrying the dexamethasone-inducible version of the MP inhibitor protein BODENLOS (GR-bdl). Without dexamethasone (DEX) treatment, this line is identical to the wild-type, while DEX treatment leads to strong inhibition of ARF-dependent transcription. To remove all endogenous MP-inhibiting Aux/IAA proteins, we treated mp or GR-bdl seedlings during 1 hour with auxin (50 micromolar Indole-3-Acetic Acid), either with or without a pretreatment with 10 micromolar DEX for 1 hour. Genes that are activated by MP are expected to br downregulated in mp seedlings and in the GR-bdl line afer DEX treatment. We used biological duplicates for each of the three treatments.
Date made available1 Apr 2010
PublisherWageningen University

Research Output

MONOPTEROS controls embryonic root initiation by regulating a mobile transcription factor

Schlereth, A., Moller, B. K., Liu, W., Kientz, M., Flipse, J., Rademacher, E. H., Schmid, M., Juergens, G. & Weijers, D., 2010, In : Nature. 464, p. 913-916

Research output: Contribution to journalArticleAcademicpeer-review

  • 332 Citations (Scopus)

    Cite this

    Schlereth, A. (Creator), Juergens, G. (Creator), Weijers, D. (Creator) (1 Apr 2010). Transcriptional profiles between mp mutant seedlings and transgenics carrying the dexamethasone-inducible GR-bdl protein. Wageningen University.