Transcription profiling of Arabidopsis or Thlaspi caerulescens roots exposed to deficient, sufficient or excess zinc

  • Judith van de Mortel (Creator)
  • E. Verloren van Themaat (Creator)
  • H. Schat (Creator)
  • J. Kwekkeboom (Creator)
  • L. Almar Villanueva (Creator)
  • Maarten Koornneef (Creator)
  • Mark Aarts (Creator)
  • P.D. Moerland (Creator)
  • S. Coughlan (Creator)

Dataset

Description

A common reference model was used to design the microarray experiment, in which cDNA from T. caerulescens roots exposed to 100 uM (sufficient) zinc was used as the common reference. Every slide was always hybridized with the common reference sample and with a sample from one of the treatments (Arabidopsis or T. caerulescens exposed to deficient, sufficient or excess zinc). The common reference was labeled with the fluorescent dye Cyanine 3 (Cy3) and the treatment samples were labeled with Cyanine 5 (Cy5). As a quality control step we performed a dye-swap hybridization for one sample (from T. caerulescens roots exposed to sufficient zinc).
Date made available10 Oct 2006
PublisherWageningen UR

Research Output

Large Expression Differences in Genes for Iron and Zinc Homeostasis, Stress Response, and Lignin Biosynthesis Distinguish Roots of Arabidopsis thaliana and the Related Metal Hyperaccumulator Thlaspi caerulescens.

van de Mortel, J. E., Almar Villanueva, L., Schat, H., Kwekkeboom, J., Coughlan, S., Moerland, P. D., Verloren van Themaat, E., Koornneef, M. & Aarts, M. G. M., 2006, In : Plant Physiology. 142, 3, p. 1127-1147

Research output: Contribution to journalArticleAcademicpeer-review

  • 326 Citations (Scopus)

    Cite this

    van de Mortel, J. (Creator), Verloren van Themaat, E. (Creator), Schat, H. (Creator), Kwekkeboom, J. (Creator), Almar Villanueva, L. (Creator), Koornneef, M. (Creator), Aarts, M. (Creator), Moerland, P. D. (Creator), Coughlan, S. (Creator) (10 Oct 2006). Transcription profiling of Arabidopsis or Thlaspi caerulescens roots exposed to deficient, sufficient or excess zinc. Wageningen UR.