Transcription profiling by array of chicken brain and ileum after infection with H7N1 HPAIV or H7N1 LPAIV

  • J. Post (Creator)

Dataset

Description

Animal experiments
Experiment : Chickens of group 1 were inoculated with 0.2 ml (2*105 EID50) of the H7N1 HPAIV strain, equally divided between the intranasal and intratracheal route. In the same way, animals of group 2 received 2*105 EID50 of the H7N1 LPAIV strain. Sampling: Six chickens from each group were sacrificed just before infection (controls) and at 4, 8, 16 and 24 h.p.i. From all sacrificed chickens, gross pathology of the organs was studied. Lung, trachea, ileum and brains were collected. For RNA isolation, organs were snap-frozen in liquid nitrogen and stored at -80C until use. For immonohistochemistry, samples were fixed immediately by immersion in a zinc salt-based fixative, containing 0.5 % zinc chloride, 0.5% zinc acetate in 0.1 M Tris base buffer containing 0.05 % calcium acetate, pH 7.4 for 48 h. After fixation, the tissues were dehydrated and embedded in paraffin wax. All HPAIV experiments were performed in Biosafety Level 3 facilities.
Date made available3 Jun 2014
PublisherWageningen UR

Research Output

Systemic Virus distribution and host responses in brain and intestine of chickens infected with low pathogenic and high pathogenic avian influenza virus

Post, J., Burt, D. W., Cornelissen, J. B. W. J., Broks, V. C. M., van Zoelen, D., Peeters, B. P. H. & Rebel, J. M. J., 2012, In : Virology journal. 9, 14 p., 61.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
  • 26 Citations (Scopus)

    Cite this

    Post, J. (Creator) (3 Jun 2014). Transcription profiling by array of chicken brain and ileum after infection with H7N1 HPAIV or H7N1 LPAIV. Wageningen UR.