Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease

  • Rhoda Delventhal (Creator)
  • R.E. Niks (Creator)

Dataset

Description

In this experiment the transcriptome reprogramming in barley during host and nonhost interaction with Puccinia sp. was analyzed in a time-series approach. Ten days old barley plants of cv. Vada were mock-inoculated or inoculated with P. hordei (Ph), 1.2.1 isolate, or P. triticina (Pt), BRW96258 isolate. After 12, 24, 36 and 48 hours first leaves were sampled. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), the Ambion TURBO DNA-free DNase Kit was used for DNA elimination, and RNA was hybridized to Agilent 44k oligonucleotide arrays.
Date made available2 Dec 2017

Research Output

A comparative analysis of nonhost resistance across the two Triticeae crop species wheat and barley

Delventhal, R., Rajaraman, J., Stefanato, F. L., Rehman, S., Aghnoum, R., McGrann, G. R. D., Bolger, M., Usadel, B., Hedley, P. E., Boyd, L., Niks, R. E., Schweizer, P. & Schaffrath, U., 4 Dec 2017, In : BMC Plant Biology. 17, 1, 232.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
  • 8 Citations (Scopus)

    Cite this

    Delventhal, R. (Creator), Niks, R. E. (Creator) (2 Dec 2017). Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease