The effect of insulin, glucose and rosiglitazone on mouse 3T3-L1 mature adipocytes gene expression

    Dataset

    Description

    Obesity is associated with insulin resistance and type 2 diabetes, implying a role for adipose tissue in the etiology of these disorders. We used cDNA microarrays to obtain expression profiles of adipocyte by the treatment of insulin and anti-diabetic drug rosiglitazone at both high and low glucose level. Mouse 3T3-L1 mature adipocytes were treated 2 days 1) with or without 1 µM insulin at low and high glucose level, 2) with or without 0.5 μM rosiglitazone at low and high glucose and insulin level. 1) The comparison between the insulin and non-insulin treatment at the same glucose level showed minor changes in the transcriptome. The expressions of some processing enzymes for extracellular matrix components were regulated by insulin. 2) Transcriptome data indicates that an altered energy metabolism is induced by rosiglitazone in mature adipocytes. In addition, rosiglitazone represses adipokine expression, except adiponectin and ApoE. Moreover, transcriptome changes indicate that a general repression of secreted protein encoding genes occurs.
    Date made available21 Nov 2006
    PublisherRIKILT / Maastricht University

    Research Output

    Absence of an adipogenic effect of rosiglitazone on mature 3T3-L1 adipocytes: increase of lipid catabolism and reduction of adipokine expression

    Wang, P., Renes, J., Bouwman, F., Bunschoten, A., Mariman, E. & Keijer, J., 2007, In : Diabetologia. 50, 3, p. 654-665

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
  • 46 Citations (Scopus)

    Cite this

    Wang, P. (Creator), Mariman, E. (Creator), Bunschoten, A. (Creator), Keijer, J. (Creator) (21 Nov 2006). The effect of insulin, glucose and rosiglitazone on mouse 3T3-L1 mature adipocytes gene expression . RIKILT / Maastricht University.