The ATP-P2X7 signaling axis is dispensable for obesity-associated inflammasome activation in adipose tissue

  • Shengyi Sun (Creator)
  • Sheng Xia (Creator)
  • Yewei Ji (Creator)
  • Sander Kersten (Creator)
  • Ling Qi (Creator)

Dataset

Description

Inflammasome activation in adipose tissue has been implicated in obesity-associated insulin resistance and type 2 diabetes. However, when and how inflammasome is activated in adipose tissue remains speculative. Here we test the hypothesis that extracellular ATP, a potent stimulus of inflammasome in macrophages via purinergic receptor P2X, ligand-gated ion channel, 7 (P2X7), may play a role in inflammasome activation in adipose tissue in obesity. Our data show that inflammasome is activated in adipose tissue upon 8-week feeding of 60% HFD, coinciding with the onset of hyperglycemia and hyperinsulinemia as well as the induction of P2X7 in adipose tissue. Unexpectedly, P2X7-deficient animals on HFD exhibit no changes in metabolic phenotypes, nor in inflammatory responses or inflammasome activation when compared to the wildtype controls. Similar observations have been obtained in hematopoietic cell-specific P2X7-deficient animals generated by bone marrow transplantation. Thus, we conclude that inflammasome activation in adipose tissue in obesity coincides with the onset of hyperglycemia and hyperinsulinemia, but unexpectedly, is not mediated by the ATP-P2X7 signaling axis. The nature of the inflammasome-activating danger signal(s) in adipose tissue in obesity remains to be characterized.
Date made available24 Feb 2012
PublisherWageningen University

Research Output

The ATP-P2X7 signalling axis is dispensable for obesity-associated inflammasome activation in adipose tissue

Sun, S., Xia, S., Ji, Y., Kersten, A. H. & Qi, L., 2012, In : Diabetes. 61, 6, p. 1471-1478

Research output: Contribution to journalArticleAcademicpeer-review

  • 46 Citations (Scopus)

    Cite this

    Sun, S. (Creator), Xia, S. (Creator), Ji, Y. (Creator), Kersten, S. (Creator), Qi, L. (Creator) (24 Feb 2012). The ATP-P2X7 signaling axis is dispensable for obesity-associated inflammasome activation in adipose tissue. Wageningen University.