Temperature-dependent alternative splicing of FLM controls flowering in Arabidopsis thaliana

  • D. Posé (Creator)
  • D.S.L. Verhage (Creator)
  • L. Yant (Creator)
  • J. Mathieu (Creator)
  • Gerco Angenent (Creator)
  • Richard Immink (Creator)
  • M. Schmid (Creator)



How plants control the transition to flowering in response to ambient temperature is only beginning to be understood. In Arabidopsis thaliana, the MADS-box transcription factor genes FLOWERING LOCUS M (FLM) and SHORT VEGETATIVE PHASE (SVP) have key roles in this process. FLM is subject to temperature-dependent alternative splicing, producing two splice variants, FLM-β and FLM-δ, which compete for interaction with the floral repressor SVP. The SVP/FLM-β complex is predominately formed at low temperatures and prevents precocious flowering. In contrast, the competing SVP FLM-δ complex is impaired in DNA binding and acts as a dominant negative activator of flowering at higher temperatures. Our results demonstrate the importance of temperature-dependent alternative splicing in modulating the timing of the floral transition in response to environmental change.

Overall design ChIP-seq A. thaliana FLM (3 replicates for gFLM and 2 replicates for FLM splice variants)
Date made available31 May 2016
PublisherMax Planck Institute for Developmental Biology


  • Arabidopsis thaliana

Accession numbers

  • GSE48082
  • PRJNA208886

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