Supplementary data: Responses to combined abiotic and biotic stress in tomato are governed by stress intensity and mechanism of resistance

Dataset

Description

Stress conditions in agricultural ecosystems can occur in variable intensities. Different resistance mechanisms to abiotic stress and pathogens are deployed by plants. Thus, it is important to examine plant responses to stress combinations under different scenarios. Here, we evaluated the effect of different levels of salt stress ranging from mild to severe (50, 100 and 150mM NaCl) on powdery mildew (PM) resistance and overall performance of tomato introgression lines with contrasting levels of partial resistance, as well as isogenic lines carrying the PM resistance genes Ol-1 (associated with slow Hypersensitivity Response; HR), ol-2 (a mlo mutant associated with papilla formation) and Ol-4 (a R gene associated with fast HR). PM resistance was affected by salt stress in a genotype and stress intensity dependent manner. In susceptible and partial resistant lines, increased susceptibility was observed under mild salt stress (50mM) which was accompanied with accelerated cell death-like senescence. On the contrary, severe salt stress (150mM) reduced disease symptoms. Na+ and Cl- accumulation in the leaves was linearly related to the decreased pathogen growth under severe stress, suggesting a more direct role for the salt in suppressing PM growth. In contrast, complete resistance mediated by ol-2 and Ol-4 was unaffected under all treatment combinations, and was associated with a decreased growth penalty. Increased susceptibility and senescence under combined stress of the variety Moneymaker (MM) and the NIL Ol-1 was associated with the induction of ethylene and jasmonic acid pathway genes as well as of the cell wall invertase gene LIN6. These results highlight the significance of stress severity and resistance type on the plant’s performance under abiotic and biotic stress combination.
Date made available2016
PublisherWageningen UR
Temporal coverage2012 - 2014

Research Output

  • 1 internal PhD, WU

Genetics and regulation of combined abiotic and biotic stress tolerance in tomato

Kissoudis, C., 2016, Wageningen: Wageningen University. 212 p.

Research output: Thesisinternal PhD, WU

Open Access
  • Datasets

    Supplementary data: Roles and contribution of tomato WRKY genes to salt stress and powdery mildew resistance

    Kissoudis, C. (Creator), Gao, D. (Creator), Pramanik, D. (Creator), Birhanu, M. (Creator), van de Wiel, C. (Creator), Visser, R. (Creator), Bai, Y. (Creator) & van der Linden, G. (Creator), Wageningen UR, 2016

    Dataset

    Supplementary data: Combined biotic and abiotic stress resistance in tomato

    Kissoudis, C. (Creator), Chowdhury, R. (Creator), van Heusden, S. (Creator), van de Wiel, C. (Creator), Finkers, R. (Creator), Visser, R. (Creator), Bai, Y. (Creator) & van der Linden, G. (Creator), Wageningen UR, 2016

    Dataset

    Cite this

    Kissoudis, C. (Creator), Sri Sunarti, S. (Creator), van de Wiel, C. C. M. (Creator), Visser, R. G. F. (Creator), van der Linden, C. G. (Creator), Bai, Y. (Creator) (2016). Supplementary data: Responses to combined abiotic and biotic stress in tomato are governed by stress intensity and mechanism of resistance. Wageningen UR. 10.18174/369637