Research Output per year
We studied the effect of dietary fat type, varying in polyunsaturated/saturated fatty acid ratio's (P/S) on development of metabolic syndrome. C57Bl/6J mice were fed purified high-fat diets (45E% fat) containing palm oil (HF-PO; P/S 0.4), olive oil (HF-OO; P/S 1.1) or safflower oil (HF-SO; P/S 7.8) for 8 weeks. A low-fat palm oil diet (LF-PO; 10E% fat) was used as a reference. Additionally, we analyzed diet-induced changes in gut microbiota composition and mucosal gene expression. The HF-PO diet induced a higher body weight gain and liver triglyceride content compared to the HF-OO, HF-SO or LF-PO diet. In the intestine, the HF-PO diet reduced microbial diversity and increased the Firmicutes/Bacteroidetes ratio. Although this fits a typical obesity profile, our data clearly indicate that an overflow of the HF-PO diet to the distal intestine, rather than obesity itself, is the main trigger for these gut microbiota changes. A HF-PO diet-induced elevation of lipid metabolism-related genes in the distal small intestine confirmed the overflow of palm oil to the distal intestine. Some of these lipid metabolism-related genes were previously already associated with the metabolic syndrome. In conclusion, our data indicate that saturated fat (HF-PO) has a more stimulatory effect on weight gain and hepatic lipid accumulation than unsaturated fat (HF-OO and HF-SO). The overflow of fat to the distal intestine on the HF-PO diet induced changes in gut microbiota composition and mucosal gene expression. We speculate that both are directly or indirectly contributive to the saturated fat-induced development of obesity and hepatic steatosis.
|Date made available||18 Jun 2012|
Saturated fat stimulates obesity and hepatic steatosis and affects gut microbiota composition by an enhanced overflow of dietary fat to the distal intestinede Wit, N. J. W., Derrien, M., Bosch-Vermeulen, H., Oosterink, E., Keshtkar, S., Duval, C. N. C., de Vogel-van den Bosch, H. M., Kleerebezem, M., Muller, M. R. & van der Meer, R., 2012, In : American Journal of Physiology. Gastrointestinal and Liver Physiology. 303, 5, p. G589-G599
Research output: Contribution to journal › Article › Academic › peer-review
de Wit, N. (Creator), Oosterink, E. (Creator), Bosch-Vermeulen, H. (Creator), Keshtkar, S. (Creator), Duval, C. N. C. (Creator), de Vogel-van den Bosch, J. (Creator), Muller, M. (Creator), van der Meer, R. (Creator) (18 Jun 2012). Saturated fat stimulates obesity and hepatic steatosis and affects gut microbiota composition by an enhanced overflow of dietary fat to the distal intestine. Wageningen University.