Role of small intestine in the development of dietary fat-induced obesity and insulin resistance in C57BL/6J mice.

Dataset

Description

Obesity and insulin resistance are two major risk factors underlying the metabolic syndrome. To gain more insight in the role of the small intestine in the etiology of these metabolic disorders, a microarray study was performed on small intestines (SI) of C57BL/6J mice that were fed a high fat diet mimicking the fatty acid composition of a Western-style human diet. The mice became obese and developed dietary fat-induced glucose intolerance. For gene expression profiling, the small intestines were subdivided in three equal parts along the longitudinal axis. The most pronounced effects of dietary fat were detected in part 2 of the small intestine. The biological processes that were most extensively modulated on a high fat diet were related to lipid metabolism, especially β- and ω-fatty acid oxidation seemed to play an important role, cell cycle and inflammation/immune response. An additional secretome analysis revealed differentially expressed secreted proteins, such as Il18, Ffgf15, Mif, Igfbp3 and Angptl4, which might provoke systemic effects in peripheral organs by influencing their metabolic homeostasis. Furthermore, many of the dietary fat-modulated genes and biological processes in small intestine were previously already associated with obesity and/or insulin resistance. Together, the data of this exploratory study provided various leads for an essential role of the small intestine in development of obesity and/or insulin resistance.
Date made available13 May 2008
PublisherWageningen University

Research Output

Supplementary dietary calcium stimulates faecal fat and bile acid excretion, but does not protect against obesity and insulin resistance in C57BL/6J mice

de Wit, N. J. W., Bosch-Vermeulen, H., Oosterink, E., Müller, M. R. & van der Meer, R., 2011, In : British Journal of Nutrition. 105, 7, p. 1005-1011

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
  • 9 Citations (Scopus)

    A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine

    van den Bosch, H. M., de Wit, N. J. W., Hooiveld, G. J. E. J., Vermeulen, H., van der Veen, J. N., Houten, S. M., Kuipers, F., Müller, M. R. & van der Meer, R., 2008, In : American Journal of Physiology. Gastrointestinal and Liver Physiology. 294, 5, p. G1171-G1180

    Research output: Contribution to journalArticleAcademicpeer-review

  • 43 Citations (Scopus)

    The role of the small intestine in the development of dietary fat-induced obesity and insulin resistance in C57BL/6J mice

    de Wit, N. J. W., Vermeulen, J. J., de Groot, P. J., Hooiveld, G. J. E. J., Grootte Bromhaar, M. M., Jansen, J., Müller, M. R. & van der Meer, R., 2008, In : BMC Medical Genomics. 1, 16 p., 14.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
  • Cite this

    de Wit, N. (Creator), Bosch-Vermeulen, H. (Creator), de Groot, P. (Creator), Hooiveld, G. (Creator), Grootte Bromhaar, M. (Creator), Jansen, J. (Creator), Muller, M. (Creator), van der Meer, R. (Creator) (13 May 2008). Role of small intestine in the development of dietary fat-induced obesity and insulin resistance in C57BL/6J mice. Wageningen University.