RNA-guided RNA silencing by an 1 Asgard archaeal Argonaute

  • Carolien Bastiaanssen (Creator)
  • Pilar Bobadilla Ugarte (Creator)
  • Kijun Kim (Creator)
  • Giada Finocchio (Creator)
  • Yanlei Feng (Creator)
  • Todd A. Anzelon (Creator)
  • Stephan Köstlbacher (Creator)
  • Daniel Tamarit (Utrecht University) (Creator)
  • Thijs Ettema (Creator)
  • Martin Jinek (Creator)
  • Ian J. MacRae (Creator)
  • Chirlmin Joo (Creator)
  • Daan Swarts (Creator)
  • Fabai Wu (Creator)

Dataset

Description

The dataset contains small RNAs that associated with HrAgo1 during heterologous expression in E. coli. The goal of the study was to determine what type of small RNAs associate with HrAgo1 and from what RNA transcripts these small RNAs are derived Overall design: His-MBP-tagged-HrAgo1 was heterologously expressed under the T7 promoter in E. coli strain BL21-Gold (DE3). His-MBP-tagged-HrAgo was purified using niNTA purification and size-exclusion chromatography. Nucleic acids were extracted from HrAgo1 and a small RNA library was generated and sequenced by GenomeScan (leiden, NL). Detailed methods are given in the associated publication.
Date made available15 May 2024
PublisherWageningen University & Research

Keywords

  • e. coli
  • RNA

Accession numbers

  • PRJNA1111834
  • GSE267550

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