Reverse genetics using CRISPR-Cas9 in the tropical tree Parasponia andersonii revealed a promotive role for PanNODULE ROOT1 in stem secondary growth

NODULE ROOT (NOOT), BLADE-ON-PETIOLE (BOP), COCHLEATA (COCH)-LIKE (NBCL) are plant-specific developmental regulator participating in many developmental process of the primary growth. NBCL contribute to the meristem-to-organ-boundaries maintenance by inhibiting meristematic activities and promoting adjacent tissues initiation, development and determinacy. To determine if NBCL contribute to the regulation of tree secondary growth, we studied the impact of the PanNODULE ROOT1 (PanNOOT1) gene loss-of-function on the secondary growth of Parasponia andersonii (P. andersonii).