Replacing the Calvin cycle with the reductive glycine pathway in Cupriavidus necator

  • N.J.H.P. Claassens (Creator)
  • Guillermo Bordanaba-Florit (Creator)
  • Charles A.R. Cotton (Creator)
  • Alberto de Maria (Creator)
  • Max Finger-Bou (Max Planck Institute of Molecular Plant Physiology) (Creator)
  • Lukas Friedeheim (Creator)
  • Natalia Giner-Laguarda (Creator)
  • Martí Munar-Palmer (Creator)
  • William Newell (Creator)
  • Giovanni Scarinci (Creator)
  • Jari Verbunt (Creator)
  • Stijn T. de Vries (Max Planck Institute of Molecular Plant Physiology) (Creator)
  • Suzan Yilmaz (Creator)
  • Arren Bar-Even (Creator)

Dataset

Description

These data belong to a metabolic engineering project that introduces the reductive glycine pathway for formate assimilation in Cupriavidus necator. As part of this project we performed short-term evolution of the bacterium Cupriavidus necator H16 to grow on glycine as sole carbon and energy source. Some mutations in a putiative glycine transporting systems facilitated growth, and we performed transcriptomics on the evolved strain growing on glycine. Analysis of these transcriptomic data lead us to the discovery of a glycine oxidase (DadA6), which we experimentally demonstrated to play a key role in the glycine assimilation pathay in C. necator. Overall design: Transcriptomes are performed for the evolved strain growing on glyine (in triplicate).
Date made available5 Jun 2020
PublisherWageningen University & Research

Keywords

  • Cupriavidus necator

Accession numbers

  • GSE151887
  • PRJNA637590

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