Microbe enriched dual RNA sequencing of Mycobacterium bovis BCG infecting macrophage-like THP-1 cells

Dataset

Description

We infected the human macrophage-like cell line THP-1 with the attenuated M. tuberculosis surrogate M. bovis Bacillus Calmette–Guérin (M. bovis BCG). We employed microbial enrichment combined with specific ribosomal RNA depletion to simultaneously analyze the transcriptional responses of host and pathogen during infection by dual RNA sequencing.
Date made available17 Jun 2014
PublisherWageningen University

Research Output

Comprehensive insights into transcriptional adaptation of intracellular mycobacteria by microbe-enriched dual RNA sequencing

Rienksma, R. A., Suarez Diez, M., Mollenkopf, H. J., Dolganov, G. M., Dorhoi, A., Schoolnik, G. K., Martins Dos Santos, V. A. P., Kaufmann, S., Schaap, P. J. & Gengenbacher, M., 2015, In : BMC Genomics. 16, 31 p., 34.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
  • 43 Citations (Scopus)

    Cite this

    Rienksma, R. A. (Creator), Suarez Diez, M. (Creator), Mollenkopf, H. J. (Creator), Dolganov, G. M. (Creator), Dorhoi, A. (Creator), Schoolnik, G. K. (Creator), Martins dos Santos, V. A. P. (Creator), Kaufmann, S. (Creator), Schaap, P. J. (Creator), Gengenbacher, M. (Creator) (17 Jun 2014). Microbe enriched dual RNA sequencing of Mycobacterium bovis BCG infecting macrophage-like THP-1 cells. Wageningen University.