Research output per year
Research output per year
Description
In this study, we exposed Caenorhabditis elegans wild types N2 to two concentrations of the model genotoxicants formaldehyde (HCHO), N-ethyl-N-nitrosourea (ENU), and methyl methanesulfonate (MMS).We performed a concentration-response test to determine the non-toxic concentration range for studying the transcriptional effects of compounds. In microarray experiments, we studied two concentrations (1 mM and 5 mM) for each compound and a control of M9 medium. Age synchronized worms at developmental L4 larval stage were exposed to treatment for two hours. After flash freezing the samples, RNA was isolated, labeled and hybridized on oligo microarray (Agilent) slides.
| Date made available | 23 Apr 2021 |
|---|---|
| Publisher | Wageningen University & Research |
Keywords
- Caenorhabditis elegans
- transcription profiling by array
- compound treatment design
- dose response design
Accession numbers
- E-MTAB-10264
Research output
- 1 Article
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Development of a transcription-based bioanalytical tool to quantify the toxic potencies of hydrophilic compounds in water using the nematode Caenorhabditis elegans
Karengera, A., Bao, C., Riksen, J. A. G., van Veelen, H. P. J., Sterken, M. G., Kammenga, J. E., Murk, A. J. & Dinkla, I. J. T., 20 Dec 2021, In: Ecotoxicology and Environmental Safety. 227, 112923.Research output: Contribution to journal › Article › Academic › peer-review
Open Access6 Citations (Scopus)
Datasets
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Microarray analysis of Caenorhabditis elegans exposed to three hydrophilic genotoxicants in water
Karengera, A. (Creator), Dinkla, I. J. T. (Creator) & Sterken, M. (Creator), Wageningen University & Research, 23 Apr 2021
https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-10265/
Dataset