Listeria monocytogenes SOS response determined after mitomycin C exposure

  • Stijn van der Veen (Creator)
  • S. van Schalkwijk (Creator)
  • D. Molenaar (Creator)
  • Willem de Vos (Creator)
  • Tjakko Abee (Creator)
  • M.H.J. Wells-Bennik (Creator)

Dataset

Description

The SOS response is a conserved pathway that is activated under certain stress conditions and is regulated by the repressor LexA and the activator RecA. The food-borne pathogen Listeria monocytogenes contains RecA and LexA homologs, but their roles in Listeria have not been established. In this study, we identified the SOS regulon in L. monocytogenes by comparing the transcription profiles of the wild-type strain and the ΔrecA mutant strain after exposure to the DNA damaging agent mitomycinC (MMC). The SOS response is an inducible pathway involved in DNA repair, restart of stalled replication forks, and in induction of genetic variation in stressed and stationary phase cells. It is regulated by LexA and RecA. LexA is an autoregulatory repressor which binds to a consensus sequence in the promoter region of the SOS response genes, thereby repressing transcription. A consensus LexA binding motif for L. monocytogenes has not been identified thus far. Generally, the SOS response is induced under circumstances in which single stranded DNA accumulates in the cell. This results in activation of RecA, which in turn stimulates cleavage of LexA, and ultimately in the induction of the SOS response.
Date made available25 Nov 2009
PublisherWageningen University

Research Output

The SOS response of Listeria monocytogenes is involved in stress resistance and mutagenesis

van der Veen, S., van Schalkwijk, S., Molenaar, D., de Vos, W. M., Abee, T. & Wells-Bennik, M. H. J., 2010, In : Microbiology. 156, 2, p. 374-384

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
  • 55 Citations (Scopus)

    Cite this

    van der Veen, S. (Creator), van Schalkwijk, S. (Creator), Molenaar, D. (Creator), de Vos, W. (Creator), Abee, T. (Creator), Wells-Bennik, M. H. J. (Creator) (25 Nov 2009). Listeria monocytogenes SOS response determined after mitomycin C exposure. Wageningen University.