A Clostridium beijerinckii NCIMB 8052 SpoIIE deficient mutant was engineered through a CRISPR cas9 method. A 2.379 kb fragment was removed from the spoIIE coding sequence. The SpoIIE mutant strain produced solvent and did not sporulate. Its genome was sequenced to confirm the gene disruption and screen for SNPs.
Diallo, M. (Creator), Kint, N. (Creator), Monot, M. (Creator), Collas, F. (Creator), Martin-Verstraete, I. (Creator), van der Oost, J. (Creator), Kengen, S. (Creator), Lopez Contreras, A. (Creator) (31 Aug 2020). Genome sequence of Clostridium beijerinckii NCIMB 8052 spoIIE mutant. Wageningen Food & Biobased Research.