SpoIIE deficient mutants in C. beijerinckii were generated using CRISPR-Cas9 to remove 2,4 kb within the gene coding sequence. Amongst the spoIIE mutant isolates, one could not produce solvents. The genome of this isolate was sequenced to confirm the spoIIE disruption and screen for SNPs.
Diallo, M. (Creator) (13 Sept 2020). Genome sequence of a non-solventogenic and SpoIIE deficient mutant Clostridium beijerinckii NCIMB 8052. Wageningen University and Research Centre.