Gene expression profiling in control, heat-shock, and recovery treatment in a RIL population of Caenorhabditis elegans L4 larvae

Dataset

Description

This experiment investigates the genetic architecture of gene expression (eQTL) in three different treatments in a N2xCB4856 RIL population of Caenorhabditis elegans. The goal is to identify genetic variation linked to differences in gene expression. We exposed 48 RILs per treatment to a control, heat-stress, and recovery treatment. More specifically these three conditions can be characterized as: (i) the control treatment was grown for 48 hours at 20C, (ii) the heat-stress treatment was grown for 46 hours at 20C followed by 2 hours at 35C, and (iii) the recovery treatment was grown for 46 hours at 20C, followed by 2 hours at 35C and thereafter 2 hours at 20C. Thereafter RNA was isolated, labelled and hybridized on microarray. The gene expression profiles were used for eQTL mapping.
Date made available30 May 2017
PublisherWageningen University & Research

Research Output

Transcriptome resilience predicts thermotolerance in Caenorhabditis elegans

Jovic, K., Grilli, J., Sterken, M. G., Snoek, B. L., Riksen, J. A. G., Allesina, S. & Kammenga, J. E., 10 Dec 2019, In : BMC Biology. 17, 1, 102.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
  • Contribution of trans regulatory eQTL to cryptic genetic variation in C. elegans

    Snoek, B. L., Sterken, M. G., Bevers, R. P. J., Volkers, R. J. M., van 't Hof, A., Brenchley, R., Riksen, J. A. G., Cossins, A. & Kammenga, J. E., 2017, In : BMC Genomics. 18, 15 p., 500.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
  • 11 Citations (Scopus)

    Datasets

    Contribution of trans regulatory eQTL to cryptic genetic variation in C. elegans

    Snoek, L. B. (Creator), Sterken, M. (Creator), Bevers, R. P. J. (Creator), Volkers, J. M. (Creator), van 't Hof, A. (Creator), Brenchley, R. (Creator), Riksen, J. (Creator), Cossins, A. (Creator) & Kammenga, J. (Creator), Wageningen University & Research, 29 Jun 2017

    Dataset

    Cite this

    Sterken, M. G. (Creator) (30 May 2017). Gene expression profiling in control, heat-shock, and recovery treatment in a RIL population of Caenorhabditis elegans L4 larvae. Wageningen University & Research.